【文獻標題】Enantioselective bioaccumulation and detoxification mechanisms of earthworms (Eisenia fetida) exposed to mandipropamid

【作者】Kuan Fang , Lingxi Han , Yalei Liu ,et.al

【作者單位】中國農(nóng)業(yè)科學院（Chinese Academy of Agricultural Sciences）

【文獻中引用產(chǎn)品】

蚯蚓細胞色素P450(CYP450)ELISA試劑盒 酶活

蚯蚓羧酸酯酶(CarE)ELISA試劑盒 酶活

蚯蚓（GST）ELISA試劑盒 酶活

【關鍵詞】Enantioselectivity,Mandipropamid enantiomers,Detoxification enzymes,Transcriptome sequencing,Detoxification mechanisms

【DOI】doi.org/10.1016/j.scitotenv.2021.149051

【影響因子(IF)】10.5

【出版期刊】《Science of the Total Environment》

【產(chǎn)品原文引用】

2.4. Determination of detoxification enzyme activities and the secondgeneration integrated biomarker response index (IBRv2)

One earthworm was randomly selected from each beaker, weighed,and homogenized with a 50 mM phosphate buffer (pH 7.8; 1:10, W/V). The homogenized mixture was then centrifuged in a cryogenic centrifuge at 9000 rpm for 10 min, and the collected supernatant was analyzed to determine the activities of CYP450, CarE, and GST using a kit provided by ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The absorbance (optical density value) was determined at a wavelength of 450 nm using a microplate reader, and the activities of CYP450, CarE, and GST in the sample were calculated from the standard curve.

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